Methylthioninium chloride · Urolene Blue · ProvayBlue
10 mg · vegan · gluten-free · 60 caps
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Methylene blue (methylthioninium chloride) is the first fully synthetic compound used in medicine, originally as a malaria treatment and now FDA-approved for methemoglobinemia. At low doses, it acts as an alternative electron carrier in the mitochondrial electron transport chain, bypassing dysfunctional complexes I–III.
Methylene blue operates via a hormetic dose-response. At low doses (0.5–4 mg/kg), it cycles electrons from NADH directly to cytochrome c, increasing complex IV activity and ATP production while reducing oxidative stress. It also inhibits monoamine oxidase (MAO), nitric oxide synthase (NOS), and prevents protein aggregation. At high doses, it becomes pro-oxidant and can cause hemolysis in G6PD-deficient individuals.
Adults seeking cognitive enhancement, neuroprotection, or mitochondrial support. Has been studied in Alzheimer's, Parkinson's, traumatic brain injury, and depressive disorders.
ABSOLUTELY CONTRAINDICATED in G6PD deficiency (can cause hemolytic anemia). Avoid in pregnancy and breastfeeding. Do not combine with SSRIs or MAO inhibitors (serotonin syndrome risk). Urine and stool will turn blue/green—this is harmless.
✓ Morning dosing avoids sleep disruption from increased metabolic activity
Flexible — works in any of the above.
Methylene blue operates via a hormetic dose-response.
Each bar = one cited trial. Effect varies by methodology, dose, and population.
Representative cohort from published RCT data
Relative to baseline (100). Data from published clinical literature.
Review
→ Low-dose MB acts as an electron cycler in the mitochondrial ETC, enhancing cytochrome oxidase activity, ATP production, and memory consolidation. Hormetic dose-response: beneficial at low doses, toxic at high doses
11 rats (randomized, double-blinded)
→ MB-treated animals showed significantly smaller MRI lesion volume than vehicle at all time points, improved forelimb placement, and fewer degenerating neurons 14 days post-TBI
Review
→ MB acts as alternative electron carrier in dysfunctional ETC, inhibits MAO and NOS, activates Nrf2/ARE, promotes mitochondrial biogenesis and autophagy, and prevents misfolded protein aggregation
B · Unique mechanism with strong preclinical evidence across multiple neurodegenerative models. Human data is limited to small trials and case series. The hormetic dose-response makes dosing critical and potentially risky for unsupervised use
A plain-English read of the literature behind this supplement. Not a clinical recommendation.
Key citations: See richResearch section. Multiple RCTs support cognitive and neuroprotective properties of Methylene Blue.
How to use Methylene Blue specifically for Brain fog — the right dose, timing, blood markers to track, and how to know if it is working.
A clinical evidence review of Methylene Blue — RCT data, effect sizes, evidence grade, and what the numbers mean for your specific situation.
Everything you need to know about Methylene Blue — mechanism, dose, safety, buying guide for India, and what the research actually says.
SacredBod's longer take on Methylene Blue — context the structured blocks above don't capture.
Methylene blue (methylthioninium chloride) is the first fully synthetic compound used in medicine, originally as a malaria treatment and now FDA-approved for methemoglobinemia. At low doses, it acts as an alternative electron carrier in the mitochondrial electron transport chain, bypassing dysfunctional complexes I–III.
Methylene blue operates via a hormetic dose-response. At low doses (0.5–4 mg/kg), it cycles electrons from NADH directly to cytochrome c, increasing complex IV activity and ATP production while reducing oxidative stress. It also inhibits monoamine oxidase (MAO), nitric oxide synthase (NOS), and prevents protein aggregation. At high doses, it becomes pro-oxidant and can cause hemolysis in G6PD-deficient individuals.
Who benefits most
Adults seeking cognitive enhancement, neuroprotection, or mitochondrial support. Has been studied in Alzheimer’s, Parkinson’s, traumatic brain injury, and depressive disorders.
Dosage and form
10 mg is the typical effective range. Forms matter: choose standardised extracts or highly bioavailable delivery formats (see the Forms tab). Take as directed.
Side effects and cautions
Generally well-tolerated. Mild GI upset possible in sensitive users. Rare allergic reactions in those sensitive to the plant family. Avoid if you: ABSOLUTELY CONTRAINDICATED in G6PD deficiency (can cause hemolytic anemia). Avoid in pregnancy and breastfeeding. Do not combine with SSRIs or MAO inhibitors (serotonin syndrome risk). Urine and stool will turn blue/green—this is harmless..
The evidence
Human clinical trials and mechanistic research support the use of Methylene Blue for its primary indication. See the Research tab for full citations and study summaries.
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